T4 RNA Ligase 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in ELISA. Suitable for Bacteriophage T4.
Quick Overview for T4 RNA Ligase 抗体 (ABIN7540667)
抗原
T4 RNA Ligase
适用
Bacteriophage T4
宿主
兔
克隆类型
多克隆
应用范围
ELISA
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Supplier Product No.
- 200-401-mx4
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Supplier
- Rockland
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原理
- T4 RNA Ligase Antibody
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交叉反应 （详细）
- Protein A purified T4 RNA Ligase antibody is directed against T4 RNA Ligase (E.coli)
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过滤
- Sterile filtered
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免疫原
- Anti-T4 RNA Ligase antibody was produced from whole rabbit serum produced by repeated immunizations with T4 Ligase isolated from E. coli.
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亚型
- IgG
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应用备注
- Optional[Neutralization_Dilution]: 1:13,000-1:14,000
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说明
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This protein A purified Anti-T4 RNA Ligase antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43.5 kDa in size corresponding to RNA Ligase by western blotting in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- T4 RNA Ligase
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背景
- Enterobacteria phage T4, RNA ligase 1, polyribonucleotide synthase (ATP), ribonucleic ligase,The T4 RNA Ligase enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA. This catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue. The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[RNA]. Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in the formation of the phosphodiester bond and release of the adenylate.
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基因ID
- 258717
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NCBI登录号
- NP_049839
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UniProt
- A0A7S9SW04
抗原
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